Vitamin D enhances Type I Interferon signaling
And the endotoxin connection
This is directly correlated with the Endotoxin published Substack, it will make even more sense on how and why such a simple intervention could and can solve a loooooooooot of problems at the end of this Substack. I will first cover this most recent paper in relation to SARS-CoV-2, and as usual build from there.
Vitamin D enhances type I IFN signaling in COVID-19 patients
The ability of Vitamin D (VitD) to modulate antiviral responses through induction of antimicrobial peptide is well established. However, the effect of VitD on host responses to SARS-CoV-2 is not well investigated. We here report the ability of VitD to enhance host IFN-alpha/beta (a/β) signaling both in vitro and among severe COVID-19 patients treated with VitD. Blood and saliva specimens were obtained from severe COVID-19 patients treated (43 patients), or not (37 patients), with vitD, during their stay in intensive care unit. Patients were followed up to 29 days following admission, and patient survival outcomes were collected. Higher activity levels of RIG-1/MDA-5 and JAK-STAT signaling pathways were observed with significantly higher gene and protein levels of antiviral interferon stimulating genes (ISGs) such as MX-1 and ISG-15; both in vitro, following treatment of PBMCs with vitD, and in whole blood and saliva specimens of VitD treated patients. Moreover, VitD treated patients had lower risk of all-cause mortality by day 29 compared to untreated patients (adjusted hazard ratio, 0.37, 95% confidence interval of 0.14–0.94; P = 0.038). The herein uncovered regulatory role of VitD on type I IFNs suggests the importance of insuring a normal level of VitD for the prevention and probably treatment of SARS-CoV-2 infection. Additional mechanistic studies, however, are needed to fully elucidate the antiviral effects of VitD particularly in the setting of COVID-19 infection.
I have written about the opposite of the mechanism this paper covers, how the virus and some of its proteins disrupt ISG genes. These genes are critical for your immune response against viruses and to some extent bacterial infections too. Disruption of this signaling causes a lot of problems, especially when there is a delay in response and your body compensates by flooding you with interferons and cytokines.
Both in vitro (testing the cells in a test tube basically) and by measuring the blood and saliva of Vitamin D-treated patients they found an increase in IGSs. And patients treated with Vitamin D had a lower risk of all-cause mortality. Thus the authors uncover a role for Vitamin D in regulating Type I Interferons and ISG (the same ones the virus disrupts and downregulates).
Vitamin D (VitD) has long been recognized as an essential vitamin for the skeletal system. Further evidence suggests that it also plays a major role in regulating the immune system, including immunity to viral infections15,16. Epidemiological studies have shown that VitD deficiency may confer increased risk of influenza and COVID-19 severity17, while supplementation on the other hand might prevent progression of COVID-19 or death18,19,20. in vitro studies also supported the fact that VitD has direct anti-viral effects and linked that to VitD's ability to up-regulate the anti-microbial peptides, including cathelicidin LL-37 and human beta defensin 215,21,22. Here, we have shown, in vitro and in the setting of COVID-19 hospitalized patients, that VitD's anti-viral mechanism can be linked to its ability to increase host type I IFN immunity by increasing the activities of RIG-1/MDA-5 signaling, JAK-STAT pathway, and the resultant IFN α/β signaling and ISGs production.
I want you to keep the bold part in mind, it will be important later in this Substack, otherwise, this is just one of the few synergies Vitamin D supplementation has with your immune system, the actual list is rather extensive, Vitamin D from the sun also has many immune-enhancing effects, literally charging your T Cells. There is a role for both, and I will make the case for supplementation after every infection or when any latent infection wakes up.
The authors went on to demonstrate and find VitD enhances IFN alpha and beta signaling in vitro.
Followed by measuring the same signaling in the blood of severe SARS-CoV-2 patients. With similar findings, mRNA (leading to the expression), and the levels of Vitamin D receptor found in the patients receiving VitD supplementation and here we have the dosage of it (50.000 IU once per week for 2 to 3 weeks). And some important genes were also found elevated, those being RIG-1, MDA-5, and p-IRF3.
Similar to what they found in the blood of patients, they found similar responses in the saliva of the patients, with this being the decision to also use saliva “Saliva fluid is believed to be a good source of non-invasive biomarkers for COVID-19 diagnosis and prognosis.”
The finding of this paper themselves is remarkable for outright disproving the almost 3 years old propagandized lie that Vitamin D has little to no effect on both SARS-CoV-2 or any other type of infection, and it only exists to supplement people experiencing a deficiency.
Tests to measure your blood levels of Vitamin D are cheap anywhere in the world, and free in many countries, and a short course of supplementation as early treatment is an effective way to combat any type of infection, with special interest to this one. Now, remember LL37 ? Vitamin D upregulates it, and so does Niacinamide. Cheap, and effective.
From here.
We have previously shown in microglia cultures that vitamin D3 deficiency may impair the resistance of the brain against bacterial infections [21]. Taken together, these data indicate an important role of vitamin D3 in the clearance of infections and containment of inflammation by the body’s immune cells.
50% of bronchiectasis patients were vitamin-D deficient, 43% insufficient and only 7% sufficient.
Taken together, the potential anti-infectious effects for the antibiotic-independent application of vitamin D and/or an adjunct therapy in combination with antibiotic compounds directed against infectious diseases such as tuberculosis, H. pylori infections, or skin diseases, for instance, should be considered and further investigated in more detail.
Here is an extensive article titled Vitamin D, infections and immunity I highly recommend the ones interested to read it. In the few articles I shared in the quotes above, and from the paper itself we now have a decent understanding of how Vitamin D will help you tackle, and clear infection, both viral and bacterial.
At the start of this, I said I would tell you supplementation after each infection is borderline obligatory, and now I will drive the point further, there are dozens of reasons why when vaccinated people ask me how to “fix/avoid damage” from the vaccines, my basic “stack” (group of supplements) has Vitamin D on it. Now I find it even more necessary than before.
LPS and HIV gp120 modulate monocyte/macrophage CYP27B1 and CYP24A1 expression leading to vitamin D consumption and hypovitaminosis D in HIV-infected individuals
Results: The prevalence of vitamin D deficiency (25OHD < 10 ng/ml) and vitamin D insufficiency (25OHD 10-30 ng/ml) was 31% and 57%, respectively. In univariate analysis, female sex (p = 0.01), increasing age (p = 0.05), higher highly sensitive-C reactive protein (p = 0.025), higher parathyroid hormone (PTH) (p = 0.043) and lower BMI (p = 0.04) were associated with vitamin D deficiency. In multivariate analysis, the association was still significant only for PTH (p = 0.03) and female sex (p = 0.03). Monocyte stimulation with LPS (100 ng/ml) or gp120 (1 µg/ml) significantly upregulated CYP27B1 mRNA expression. Moreover, gp120 significantly increased VDR mRNA levels. On the contrary, neither LPS nor gp120 modified CYP24A1 levels. Macrophage stimulation with LPS (100 ng/ml) significantly upregulated CYP27B1 and CYP24A1 mRNA expression. When monocytes were cultured in the presence of 25OHD (40 ng/ml) and stimulated with LPS we detected significantly lower levels of 25OHD in the supernatant.
Conclusions: Vitamin D deficiency was very common in our cohort of HIV-infected patients. Chronic inflammation, including residual viral replication, may contribute to hypovitaminosis D, by modulating vitamin D metabolism and catabolism. Systematic screening may help identifying subjects requiring supplementation.
LPS has also been found to down-regulate Vitamin D Receptors, especially in the oral mucosa.
No, I do not think this is AIDS, and I am the person responsible for literally equating the vaccines to “VAIDS”, but there are many many many “similarities” and mechanisms that are 1:1 mimics of some effects of AIDS, and have 4 HIV inserts, in which 3 ARE FUNCTIONAL.
In the paper above they showed exactly what you read, that both LPS, and GP120 (which the Spike Protein has 4 parts, 3 functional) both modulate certain genes leading to Vitamin D consumption (sometimes it is referred to as a nutrient sink, wasting said nutrient, HIV also causes Niacin/Vitamin B3 sink), ending with deficiency. Given my most recent Substack on the Spike Protein and endotoxin (LPS), anyone can quite well deduce where this is going.
Interleukin-12 has been one of my major interests in mid-2021 and the cornerstone for a few months in the effort of me and a friend to basically “reverse engineer” the mRNA vaccines. In regards to severe SARS-CoV-2 cases sometimes, and a sizable portion of vaccine injuries, long-term especially, Interleukin-12 is one of incredible importance, from multiple perspectives. Add another reason to the pile.
I think this is enough for now, but for those that would like to go further and connect to a lot of my other writings, of interest is the Kynurenine Pathway, Vitamin D has a regulatory action on the signaling pathway of Hypoxia-Inducible Factor-1a (HIF-1a), one of the most important “secrets” since the start of this pandemic. More on the subject later, most likely.
Oh, I almost forgot. The subject of my next Substack. I wish you all a good week.
As usual, big thank you to all supporters here and on Kofi, and everyone who shares my Substack !
For all new subscribers, you would do well into going read older posts, because most of my SARS-CoV-2 is one massive big “thing”.
Here is all you need to know about vitamin D if interested. https://starpower.substack.com/?r=oh1g6&utm_campaign=pub&utm_medium=web
Praise the sun, indeed. And you, too.