Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination

!!!!!

I seldom send e-mails in short sequences, unless there is a finding that bears the weight of breaking this self-imposed rule. This is one of those moments.

The title is self-explanatory, and this will be a relatively shorter e-mail, yet one of the most meaningful for this week most likely.

Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination

COVID-19 mRNA vaccines effectively reduce incidence of severe disease, hospitalisation and death. The biodistribution and pharmacokinetics of the mRNA-containing lipid nanoparticles (LNPs) in these vaccines are unknown in humans. In this study, we used qPCR to track circulating mRNA in blood at different time-points after BNT162b2 vaccination in a small cohort of healthy individuals. We found that vaccine-associated synthetic mRNA persists in systemic circulation for at least 2 weeks. Furthermore, we used transmission electron microscopy (TEM) to investigate SARS-CoV-2 spike protein expression in human leukemic cells and in primary mononuclear blood cells treated in vitro with the BNT162b2 vaccine. TEM revealed morphological changes suggestive of LNP uptake, but only a small fraction of K562 leukemic cells presented spike-like structures at the cell surface, suggesting reduced levels of expression for these specific phenotypes.

It is pretty straightforward. Authors found that vaccine mRNA persists in systemic circulation for at least 2 weeks. Both of these matter. Time, and systemic.

Vaccine mRNA Remains in Circulation for at Least 15 Days

We used qPCR to investigate the presence and persistence of synthetic mRNA in blood after one, two and three doses of BNT162b2, assuming that the biodistribution of the vaccine is similar after each dose. We also aimed to probe whether vaccine mRNA was associated with circulating WBCs, either as a result of cell–LNP interactions at the injection site and in draining LNs or of random collisions in circulation. Consequently, we separated the plasma and cellular fractions and analyzed them independently for all individuals.

Template copy numbers decreased with the day of sampling. In plasma, mRNA was immediately detectable at just hours following vaccination, remained detectable when sampled at 6 and 15 days (Figure 1A, green), but was below the limit of quantification (LoQ) for one sample at 27 days. Samples from negative controls did not amplify. For subject B3, we observed a similar trend for plasma: vaccine-associated mRNA became detectable immediately after vaccination and remained significantly above the LoQ at day 14 (Figure 1B, green). Interestingly, vaccine mRNA was detected in the cellular fraction up to day 6 in some samples from our cohort, whereas for B3, it was only detectable at one day after vaccination (Figure 1A,B, orange). It has to be noted that the likelihood of detecting vaccine mRNA in the cellular fraction decreased significantly at 24 h from injection, which was in contrast to plasma in which mRNA remained consistently detectable up to day 15 (Figure 1C).

The vaccine mRNA is uptaken by your cells pretty fast, they could not find detectable levels of it in the cellular fraction of the samples, meaning it did what it was supposed to do, but they found it abundantly in plasma for up to 15 days, which we could expect in some people may last even longer.

Changes in the cells and how they observed their hypothesis…

Our results show extended plasma clearance times compared to estimates presented by mRNA vaccine manufacturers. Data provided by Pfizer-BioNTech showed luciferase-encoding RNA constructs containing proprietary ionisable lipids were terminally cleared in the plasma of rats after a maximum of 6 days. Similarly, Moderna LNP-mRNA constructs became undetectable in the plasma of rats within one day, as measured by a multiplex branched DNA assay

We detected vaccine mRNA overwhelmingly in the plasma fraction, suggesting it mostly circulates freely and not actively transported by WBCs. Moreover, it was likely circulating in its lipid-encapsulated form, as naked mRNA would have been rapidly degraded in the extracellular environment. We therefore expect these particles to also retain their ability to induce S-protein expression in susceptible cells over this time period, and we infer that such low-level, prolonged antigenic stimulation may contribute to effective immune responses, especially the proliferation of memory T-cells

First, and something we all knew by now, the manufacturers did cut corners, jumped steps, ignored all and any safety signals, and outright lied to governments, regulatory agencies, and the public. No surprise there.

The surprising part was the conclusion by the authors. The mRNA encapsulated inside its Lipid Nanoparticles floats around your body, freely, for up to 2 weeks, being able to express itself when the correct cells pick it up and transcribe it (simplified terms, like receiving code and executing it). But that part that jumped to my eyes almost instantly was the last one.

“prolonged antigenic stimulation”

This explains a lot of my questions, a lot of aspects I looked into too, a lot of the (weird) reactions, the rashes sometimes, the worsening of Long Covid. Persistent, prolonged antigenic stimulation (it means making your immune system keep working producing a response) is not something you want in regards to this specific virus.

It explains a lot of the things here. One of my major observations.

Bio Briefing - Spikes everywhere