SARS-CoV-2 mRNA vaccine suppresses red blood cell formation
"cutting to the bone"
I am now back home, everyone got sick, except me. The mobile internet didn’t collaborate until I got back home, and while the main goal today was sharing pictures and some reflections, important information or corroboration takes precedence.
As a reminder, I never edit published articles (unless it is to correct gross grammatical or syntactic errors), so at the point in time when I published both referred substacks below, Endotoxin/LPS interaction with the Spike Protein and vaccines were merely another “insane” observation in my mind. An obvious shift can be easily tracked in the second article referenced below, I will interject more later on, to avoid causing substantial confusion to an already complicated subject.
For simplicity's sake. HSPCs (Hematopoietic Stem and Progenitor Cells) are like the "building blocks" of your blood. They're special cells in your body that have incredible ability – they can turn into different types of blood cells, like red blood cells that carry oxygen, white blood cells that fight infections, and platelets that help with blood clotting.
One aspect of both the viral infection and mostly the vaccination kept in my mind for months was the effects of the virus/Spike Protein on Hematopoietic Stem/Progenitor Cells (HSPC) and the thymus, with the first arguably being among the most important cells we have.
The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
This study was done in a cell line widely used to study, among other subjects, immune responses, globin gene expression, and erythropoiesis (the process by which new red blood cells (RBC) are formed from hematopoietic stem cells, erythrocytes are the fancy word for RBC) and globin gene expression, as the name implies is the finding the genes responsible for producing globin proteins. Globin proteins, as the names indicate, are the primary parts of hemoglobins.
The first step was testing the effect of the Pfizer mRNA vaccine on the proliferation of K562 cells, in which 0.5 µg/mL was sufficient to cause inhibition of cell growth of the cells, also as expected the cells produced Spike Protein, increased expression of NF-kB was found in K562 cells.
When cells are exposed to the BNT162b2 vaccine, full inhibition of expression of α-globin (Figure 2A), γ-globin (Figure 2B) ε-globin (Figure 2C) and ζ-globin (Figure 2D) genes was obtained and correlates with a sharp enhancement of Spike mRNA content. The response to the low BNT162b2 concentration (0.5 μg/mL) is to some extent surprising, since indicates and apparent increase of globin gene expression. This should be further studied and might be due to the non-RNA constituent(s) of the BNT162b2 vaccine.
Following this, the authors tested if the mRNA vaccine was able to suppress the differentiation of erythroid cells (the steps needed to become fully functional red blood cells, in a simplistic manner of describing). First tested was mithramycin (MTH), and the mRNA vaccine was able to inhibit the differentiation, this was highly reproducible in their tests, followed by testing other common inducers, such as rapamycin, hydroxyurea, resveratrol, and isoxazole analogue C4, and once again, treating the K cells with the mRNA suppresses hemoglobin accumulation.
Here they demonstrated that Pfizer’s mRNA vaccine causes a dose-dependent inhibition of the intracellular content of the mithramycin-induced d zeta-globin, alpha-globin, epsilon globin, and gamma-globin mRNAs. These are the genes that will create the globins that are part of hemoglobins. Using 0.5 µg/mL (micrograms) inhibited the expression of these genes on average by 60%, at 1 µg/mL the average jumps to 90%.
The last section of this paper is one of the subjects I seldom cover…
These data are fully in agreement with those presented in Figure 2 and Figure 5 and demonstrate that the BNT162b2 vaccine strongly inhibits accumulation of embryo-fetal globins.
Interestingly, the expression of other genes involved in erythroid differentiation, such as the transferrin receptor gene was not affected by BNT162b2 treatment (Figure 7), suggesting that the inhibition of the expression of globin genes in K562 cells treated with BNT162b2 is specific.
Even though this study was done in a (fairly specific) cell line, there are many clinical and researcher papers written on the effects of SARS-CoV-2 on the pathway analyzed here, and similar to the referenced substacks, this adds another layer and further proof to the effects of the virus and especially mRNA-Spike Protein in an extraordinarily important pathway.
Given the importance of HSPC and erythroid pathways to both immune and physiological function, they are prone to respond to inflammation quickly, HSPC especially is very sensitive to acute, “large” inflammatory responses. So we need to clarify certain aspects of this mRNA-induced mess.
At this point, most people interested in Covid and the horrendous performance of mRNA vaccines are aware they are contaminated with many toxic materials, with the most pertinent to our interests being Endotoxins (LPS), and to this effect, adding salt to injury, the Spike Protein with its Proline substitutions becomes a magnet to the same. So in the rare case one gets a “purer” batch, the mRNA-produced Spike is gathering endotoxins from anywhere it comes in contact with an LPS source.
To that effect, both the pathway discussed here (NF-Kb by the vaccine), and in the other substacks are also shared by LPS. In fact, HSPCs are incredibly susceptible to responses to TLR-4, the main receptor for LPS/Endotoxin. One of the mechanisms discussed is by the known inflammasome NLRP3, which also participates in much of the damage and pathways of Endotoxin-induced damage. The presence of chronic long-term inflammation or (persistent) endotoxemia (endotoxin in the blood) will both drive HSPCs to exhaustion and contribute heavily towards inflammaging.
Perhaps now you are aware of my focus on controlling any level of inflammation, especially sustained inflammation, and to that effect, NAC and Punicalin supplementation (a pomegranate-derived compound) both ameliorate and mitigate the specific inflammation signals and pathways discussed above. Via modulation of other mechanisms, both Metformin and Berberine do the same. I think you get what I am implying, that my usual suggested supplements can mitigate, and help recovery.
As my final note, and to add to the complexity.
Besides other insidious pathogens, our “targets” of interest as of lately can all develop latency in the very cells virus and mRNA-Spike effect.
Nothing seems to get easier and still many walk through life with a pillow taped to their head - Wake up people!!
As sad as this is, thanks for writing this. Every time I learn more, I am so deeply saddened for humanity. I worry so much about my son and his generation. What will life be like for them as they face a lifetime of exposure to the virus and spike protein being shed.